dnaK [2017-8-11 16:0:3]

class I heat-shock protein (molecular chaperone)

locus: BSU25470

pI: 4.57

mw: 65.83 kDa

protein length: 611 aa

gene length: 1833 bp

function: protein quality control

product: class I heat-shock protein (molecular chaperone)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 2,626,112 → 2,627,947

The protein

Protein family

• heat shock protein 70 family (according to Swiss-Prot)

Modification

• phosphorylated on Tyr-601 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|27148221,17726680]

Structure

• [PDB|2V7Y] (from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|18400763]
• [PDB|4ANI] ([protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK]-[protein|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|GrpE] complex from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|22544739]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• membrane-proximal (Spotty) [Pubmed|16479537]
• recruited to the membrane after ethanol stress [Pubmed|22268681]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
  description: [Pubmed|9023197]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1339421], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|1339421], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]

  regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]

Biological materials

Mutant

• BKE25470 (''dnaK''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center] [pubmed|28189581]

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]

References

• 1339421,12224648,10383760,9023197,7540247,7592421,16479537,1339421,17726680,22268681,15378759,22544739,18400763,27148221